DNA

Part:BBa_K5300041:Design

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-30)


PnuoA-mCherry-RBS-vapC-Ter-PnifH-vapB-PglnK-sgRNA-mCherry-tracrRNA-PnuoA-Cas12k-double Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2013
    Illegal SpeI site found at 1272
    Illegal PstI site found at 513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2013
    Illegal NheI site found at 1005
    Illegal SpeI site found at 1272
    Illegal PstI site found at 513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2013
    Illegal BglII site found at 1915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2013
    Illegal SpeI site found at 1272
    Illegal PstI site found at 513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2013
    Illegal SpeI site found at 1272
    Illegal PstI site found at 513
    Illegal NgoMIV site found at 1394
    Illegal AgeI site found at 5
    Illegal AgeI site found at 1601
    Illegal AgeI site found at 3797
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing circuits, we added terminators derived from the Sinorhizobium fredii CCBAU45436 genome to vapC, vapB, and sgRNA-mCherry to ensure that regulation proceeds as expected.


Source

Sinorhizobium fredii CCBAU45436, Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110), Scytonema hofmannii.

References